Abstract
The importance of leaf rolling in rice (Oryza sativa L.) has been widely recognized. Although several studies have investigated rice leaf rolling and identified some related genes, knowledge of the molecular mechanism underlying rice leaf rolling, especially outward leaf rolling, is limited. Therefore, in this study, differential proteomics and gene expression profiling were used to analyze rolled leaf mutant of transgenic rice in order to investigate differentially expressed genes and proteins related to rice leaf rolling. To this end, 28 differentially expressed proteins related to rolling leaf traits were isolated and identified. Digital expression profiling detected 10 genes related to rice leaf rolling. Some of the proteins and genes detected are involved in lipid metabolism, which is related to the development of bulliform cells, such as phosphoinositide phospholipase C, Mgll gene, and At4g26790 gene. The “omics”-level techniques were useful for simultaneously isolating several proteins and genes related to rice leaf rolling. In addition, the results of the analysis of differentially expressed proteins and genes were closely consistent with those from a corresponding functional analysis of cellular mechanisms; our study findings might form the basis for further research on the molecular mechanisms underlying rice leaf rolling.
Highlights
Rice (Oryza sativa L.) is one of the most important grain crops worldwide, in populous countries such as China
After screening, 34 differentially expressed protein spots that were not related to the rolled leaf trait were obtained (S1 Table and Fig 1)
Our results suggested that Mgll genes, encoding monoglyceride lipase, and At4g26790, Genetic and proteomic analysis of the Oryza sativa L. rolled leaf mutant encoding GDSL lipase, might be involved in tonoplast morphogenesis, thereby potentially playing a role in bulliform cell development
Summary
Rice (Oryza sativa L.) is one of the most important grain crops worldwide, in populous countries such as China. Under conditions of limited farmland, stabilizing and improving rice yield per unit area is the most effective way to improve overall rice yields. Improving the quality of rice plant is important for increasing the unit yield of rice. The erectness of rice leaves and their proper rolling are important factors that determine ideal type of rice plants [1,2]. Proper inward rolling of the leaves enables them to be upright and not drooping, thereby reducing mutual shielding between the leaf blades and improving the light transmittance (transmission efficiency) of the community [3]. Several studies have been focusing on the rice leaf rolling trait, the isolation of genes related to leaf rolling and the investigation of the mechanisms underlying rice leaf rolling
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