Abstract
“Zhu She Yong Xue Shuan Tong” lyophilized powder (ZSYXST), consists of a series of saponins extracted from Panax notoginseng, which has been widely used in China for the treatment of strokes. In this study, an ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) combined with preparative high performance liquid chromatography (PHPLC) method was developed to rapidly identify both major and minor saponins in ZSYXST. Some high content components were removed through PHPLC in order to increase the sensitivity of the trace saponins. Then, specific characteristic fragment ions in both positive and negative mode were utilized to determine the types of aglycone, saccharide, as well as the saccharide chain linkages. As a result, 94 saponins, including 20 pairs of isomers and ten new compounds, which could represent higher than 98% components in ZSYXST, were identified or tentatively identified in commercial ZSYXST samples.
Highlights
“Zhu She Yong Xue Shuan Tong” lyophilized powder, containing saponins of Panax notoginseng, is commonly used for treating strokes in the clinic
There was a chemical analysis of ZSYXST by an LC/MS method, only 30 compounds were identified because the researchers only used a normal LC/MS method [7]
Combining Ultra-high performance liquid chromatography (UHPLC) with Q-TOF/MS (UHPLC-Q-TOF/MS) could be an effective method to identify the chemical constituents in ZSYXST
Summary
“Zhu She Yong Xue Shuan Tong” lyophilized powder, containing saponins of Panax notoginseng, is commonly used for treating strokes in the clinic. It can dilate blood vessels, promote blood circulation [1], and prevent thrombosis [2,3,4]. The average annual sales of ZSYXST in China are about a hundred million dollars It is widely used in China, its chemical constituents, especially the minor compounds, are not understood very well. With the help of fragmentation pathway rules and finding compounds by the molecule feature in the Agilent Mass Hunter Workstation Software (Version B.02.00), 94 saponins, including 20 pairs of isomers, which could represent more than 98% of the components were identified or tentatively identified in commercial ZSYXST samples. Based on the literature and the SciFinder database, compounds 1, 2, 18, 20, 21, 22, 50, 51, 59, 90 were speculated to be new saponins in ZSYXST
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