Analysis of the complete genome sequence of Brevibacterium frigoritolerans ZB201705 isolated from drought- and salt-stressed rhizosphere soil of maize

Chun Zhang,Xianglong Li,Longfei Yin,Cai Liu,Zhongbao Zhang,Zhongyi Wu,Huawen Zou

doi:10.1007/s13213-019-01532-0

Abstract

PurposeTo analyze the complete genome sequence of the Brevibacterium frigoritolerans ZB201705, a Brevibacterium strain was isolated from the maize rhizosphere in drought- and salt-stressed soil, and the activity of the strain under simulated drought and high salt conditions was assessed.MethodsWe used a combination of the PacBio RS and Illumina sequencing platforms to obtain the complete genome sequence of B. frigoritolerans ZB201705.ResultsThe genome consists of 5,475,560 bp in a linear chromosome with no gaps, 4,391 protein-coding sequences, 39 ribosomal RNAs, and 81 transfer RNAs. The genome analysis revealed many putative gene clusters involved in defense mechanisms. In addition, an activity analysis of the strain under high-salt and simulated drought conditions helped clarify its potential tolerance to these abiotic stresses.ConclusionsOur data revealed the complete genome sequence of the new isolated strain, and showed that it produces many proteins involved in drought and salt stress responses, suggesting that B. frigoritolerans ZB201705 may be a potential factor to increase crop yield under abiotic stresses. The information provided here on the genome of B. frigoritolerans ZB201705 provides valuable insight into rhizobacteria-mediated plant salt and drought tolerance and rhizobacteria-based solutions for agriculture under abiotic stress.

Highlights

The genus Brevibacterium was suggested by Breed (1953) based on Brevibacterium linens as the type species
Brevibacterium has been isolated from various environments such as food, humans, and saline environments, and other environment such as soil of ginseng field (Jung et al 2018)
The obtained data were subjected to an analysis of variance using the general linear model software Agres and Agdata, Organism information and classification

Summary

Introduction

The genus Brevibacterium was suggested by Breed (1953) based on Brevibacterium linens as the type species. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. College of Life Sciences, Capital Normal University, Beijing 100048, China. College of Agriculture, Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434023, Hubei, China species of Brevibacterium were isolated and studied. Brevibacterium has been isolated from various environments such as food (e.g., fermented seafood [Choi et al 2013] and cheese [Leclercq-Perlat et al 2000]), humans (e.g., clinical specimens [Wauters et al 2001] and the human body [Wauters et al 2003]), and saline environments (e.g., saline soil [Tang et al 2008], beach sediment [Lee 2006], marine environments [Lee 2008], salt-lake sediment [Guan et al 2010]), and other environment such as soil of ginseng field (Jung et al 2018)

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Conclusion