Abstract
Analysis of collagen and gelatin derived from single type fishbladder preparations of isinglass finings demonstrated that the specific optical rotation of each preparation was consistent for a given wavelength of incident light and was independent of the isinglass source. These specific rotation values in conjunction with measurement of the specific rotation of an isinglass preparation enabled the direct estimation of collagen content. Furthermore, thermal denaturation of isinglass preparations and polarimetric determination of the total soluble nitrogen content of the resulting gelatin provides a rapid and convenient alternative to Kjeldahl determination of the total soluble nitrogen content of isinglass finings. Measurement of the observed rotation of isinglass preparations and the total soluble nitrogen content of thermally denaturated aliquots of the same material by polarimetry enabled the analysis of collagen and total soluble protein content within 40 minutes compared to 24 hours for conventional quality control procedures based on dialysis of isinglass preparations.
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