Abstract
Multilocus sequence typing (MLST) based on six loci was used to analyze the relationship of 58 Candida tropicalis isolates from individual patients in a general hospital in Beijing, China. A total of 52 diploid sequence types (DSTs) were generated by the MLST, all of which were new to the central database. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrograms were constructed, which showed that the 58 isolates were distributed robustly and 6 main groups were clustered regardless of the specimen source and medical department. The minimum spanning tree (MST) of the 58 isolates (52 DSTs) and all 401 isolates (268 DSTs) in the C. tropicalis central database (http://pubmlst.org/ctropicalis/) indicated that the isolates in this study clustered in three relative pure clonal complexes, and 2 clustered with isolates from Taiwan, Belgium, Brazil, and the US. This study presents the first MLST analysis of C. tropicalis isolates from Mainland China, which may be useful for further studies on the similarity, genetic relationship, and molecular epidemiology of C. tropicalis strains worldwide.
Highlights
With the increasing number of immunocompromised patients, long-term hospitalized patients, and invasive medical inspection and therapy, the genus Candida has emerged as a major group of opportunistic pathogens that cause superficial and invasive infections in humans [1,2,3,4,5]
The aim of our study is to examine the clonality of C. tropicalis by performing successive strain collection for one year in a general hospital in Beijing, China, and to ascertain whether factors such as hospital department origin, anatomic source, and so on are related to certain specific Multilocus sequence typing (MLST) diploid sequence types (DSTs) types
C. tropicalis is considered as the leading cause of nosocomial fungemia and hepatosplenic fungal infections in patients with hematologic malignancies [13,14]
Summary
With the increasing number of immunocompromised patients, long-term hospitalized patients, and invasive medical inspection and therapy, the genus Candida has emerged as a major group of opportunistic pathogens that cause superficial and invasive infections in humans [1,2,3,4,5]. Candida is considered the fourth most commonly isolated organisms from nosocomial bloodstream infections in United States and sixth in Europe [6,7,8,9,10]. Candida albicans accounts for the majority of infections, other non-albicans Candida species such as Candida tropicalis have increasingly been recognized as pathogens for these types of infections. C. tropicalis is considered the leading pathogen in nosocomial fungemia and hepatosplenic fungal infections in patients with cancer, especially leukemia [13,14]. C. tropicalis is the second most frequently isolated non-albicans pathogen in the Asia-Pacific region and in Brazil [15,16]. A high proportion of C. tropicalis isolates has exhibited low susceptibility to flucytosine [24,25]
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