Analysis of the clinicopathological characteristics and immunophenotypic of primary systemic anaplastic large cell lymphoma

Abstract

Objective To Analysis the clinicopathology characteristics, immunophenotype of anaplastic large cell lymphoma (ALCL) in order to improve its diagnostic and therapeutic accuracy. Methods The expression of ALK, Ki-67, Caspase-3, CD2, CD3, CD4, CD5, CD7, CD20, CD15, CD30, EMA, Granzyme B,Perforation element and TIA-1 was detected in 22 cases ALCL using Immunohistochemical S-P method. The clinical, immunophenotypic and histopathologic features of 22 cases ALCL were retrospectively studied. Results All 22 cases were primary systemic ALCL. Among them, 15 cases were ALK-positive and 7 cases were ALK- negative. The patients with ALK-positive were younger than those with ALK-negative case (P ＜0.05). Immunohistochemical study showed that ALK-positive ALCL always expressed CD30 and EMA. In ALK-positive ALCL cases, the ageswas younger and the expressions of Ki-67 were lower, and the expressions of caspase-3 were higher than those of ALK-negative cases, in which the difference was statistically significant (P ＜0.05). The expression of Ki-67 was negative correlated with caspase-3 (P ＜0.05). Among the 22 cases of ALCL, 4 patients (18.2 ％) achieved a complete remission (CR) and 8 patients (36.4 ％) achieved a part remission (PR), and the rate of CR and PR was 54.5 ％ (12/22). The 1-year overall survival rate for all patients were 59.1％ (13/22). ALK, Ki-67, Caspase-3, clinical staging,LDH level and IPI score were found to be the prognostic factors associated with overall survival in ALCL. Conclusion ALCL cases with positive for ALK showed low degree of malignancy than ALCL cases with negative for ALK. The differences of clinical features, immunophenotypes between ALK-positive ALCL and ALK-negative ALCL groups are helpful in the differential diagnosis. The prognosis of ALCL is significantly correlated with ALK, Ki-67, Caspase-3, stages, level of LDH and IPI score. Key words: Lymphoma, large-cell; ALK; Immunophenotyping; Prognosis