Abstract
The structure and organization of the chicken retina has been investigated with an adaptive optics multiphoton imaging microscope in a backward configuration. Non-stained flat-mounted retinal tissues were imaged at different depths, from the retinal nerve fiber layer to the outer segment, by detecting the intrinsic nonlinear fluorescent signal. From the stacks of images corresponding to the different retinal layers, volume renderings of the entire retina were reconstructed. The density of photoreceptors and ganglion cells layer were directly estimated from the images as a function of the retinal eccentricity. The maximum anatomical resolving power at different retinal eccentricities was also calculated. This technique could be used for a better characterization of retinal alterations during myopia development, and may be useful for visualization of retinal pathologies and intoxication during pharmacological studies.
Highlights
The structure and organization of the chicken retina has been investigated with an adaptive optics multiphoton imaging microscope in a backward configuration
From the stacks of images corresponding to the different retinal layers, volume renderings of the entire retina were reconstructed
Chickens (Gallus domesticus) present several advantages as an animal model to study the mechanisms of myopia development [1]
Summary
Chickens (Gallus domesticus) present several advantages as an animal model to study the mechanisms of myopia development [1]. They have large eyes that grow surprisingly fast (100 μm per day), exhibit good optics [2,3] and powerful accommodation [4]. The chicken reaches maturity in less than a year [9]. It can be bred and maintained in the laboratory from the day of hatching. Chickens have served as animal models for the study of the cornea wound healing after refractive surgery treatments, such as LASIK, LASEK or PRK [10,11,12,13]
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