Abstract
Campylobacter jejuni is a leading bacterial cause of human gastroenteritis. The goal of this study was to analyze the C. jejuni F38011 strain, recovered from an individual with severe enteritis, at a genomic and proteomic level to gain insight into microbial processes. The C. jejuni F38011 genome is comprised of 1,691,939 bp, with a mol.% (G+C) content of 30.5%. PacBio sequencing coupled with REBASE analysis was used to predict C. jejuni F38011 genomic sites and enzymes that may be involved in DNA restriction-modification. A total of five putative methylation motifs were identified as well as the C. jejuni enzymes that could be responsible for the modifications. Peptides corresponding to the deduced amino acid sequence of the C. jejuni enzymes were identified using proteomics. This work sets the stage for studies to dissect the precise functions of the C. jejuni putative restriction-modification enzymes. Taken together, the data generated in this study contributes to our knowledge of the genomic content, methylation profile, and encoding capacity of C. jejuni.
Highlights
Campylobacter jejuni is an important pathogen causing significant morbidity and mortality
The C. jejuni F38011 strain contained 122 putative coding sequences (CDSs) with little similarity to genes found in NCTC 11168 strain; the C. jejuni F38011 strain lacked 58 CDSs present in the NCTC
We have further characterized the C. jejuni F38011 strain using Pacific Biosciences (PacBio)'s Single-Molecule RealTime (SMRT) sequencing coupled with methylome analysis
Summary
Campylobacter jejuni is an important pathogen causing significant morbidity and mortality. C. jejuni is a Gram-negative, comma-shaped, microaerophilic bacterium, and is motile by means of unipolar or bipolar flagella. The genus Campylobacter was proposed in 1963, separating these bacteria from Vibrio-like organisms based on morphology, DNA composition, microaerobic growth requirement, and non-fermentive metabolism [1]. C. jejuni was first isolated from human feces in 1972 [2]. Called campylobacteriosis, often occurs after handling or ingesting food contaminated by raw poultry products. Clinical infection with C. jejuni presents as diarrhea with blood and leukocytes, fever, nausea, and severe abdominal cramps that occur 2–5 days following ingestion [3, 4]. Infection rates with Campylobacter spp. has been comparable to or has exceeded that of other enteric pathogens, including Salmonella spp. and Shigella spp. The highest prevalence of Campylobacter-mediated disease is among children less than
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