Abstract
A new procedure for the assay of ergot is presented. Total alkaloids are extracted with ammonia-methanol-chloroform and water-soluble alkaloids are separated by column chromatography. Total and water-soluble ergot alkaloid contents are determined colorimetrically. The relative concentrations of ergonovine and ergonovinine in the water-soluble alkaloid fraction are estimated by paper chromatography. The new procedure yields higher values for water-soluble alkaloid content than does the current N. F. procedure.
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