Abstract
Standard wet chemistry analytical techniques currently used to determine plant fibre constituents are costly, time-consuming and destructive. In this paper the potential of near-infrared reflectance spectroscopy (NIRS) to analyse the contents of acid detergent fibre (ADF) in turnip greens and turnip tops has been assessed. Three calibration equations were developed: in the equation without mathematical treatment the coefficient of determination (R2) was 0.91, in the first-derivative treatment equation R2 = 0.95 and in the second-derivative treatment R2 = 0.96. The estimation accuracy was based on RPD (the ratio between the standard deviation and the standard error of validation) and RER (the ratio between the range of ADF of the validation as a whole and the standard error of prediction) of the external validation. RPD and RER values were of 2.75 and 9.00 for the treatment without derivative, 3.41 and 11.79 with first-derivative, and 3.10 and 11.03 with second-derivative. With the acid detergent residue spectrum the wavelengths were identified and associated with the ADF contained in the sample. The results showed a great potential of NIRS for predicting ADF content in turnip greens and turnip tops.
Highlights
The plants of the genus Brassica constitute one of the economically most important plant groups in the world
Acid detergent fibre (ADF) is an estimator of the content in cellulose, lignin, cutine and insoluble minerals in the cell wall and it is determined as the residue remaining after the digestion of the sample with an acid detergent solution
The treatment selected for one parameter in a dataset is not always the best option for the same parameter in any other set of samples [24]; this confirms the importance of optimizing the treatment for each parameter and dataset
Summary
The plants of the genus Brassica constitute one of the economically most important plant groups in the world. One drawback of double hydrolysis is that it solubilizes part of the hemicellulose and of the lignin of the cell wall, so that the result obtained of the crude fibre content is lower than the real content in structural carbohydrates This problem is avoided by using detergent solutions for the fibre analysis, following the method proposed by Goering and Van Soest [7]. Acid detergent fibre (ADF) is an estimator of the content in cellulose, lignin, cutine and insoluble minerals in the cell wall and it is determined as the residue remaining after the digestion of the sample with an acid detergent solution (made up of diluted sulphuric acid and cetyl-trimethyl-ammonium bromide). In order to employ them as a tool for a fast and non-destructive analysis in the screening of germplasm and in the selection of genotypes of the highest quality with respect to this component
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