Abstract
The application of supercritical fluid chromatography (SFC) with polar packed columns and light-scattering detection for the analysis of sugars is reported. Cyano-, diol- and nitro-bonded silicas were used with carbon dioxide-methanol mobile phases and a comparison of sugar retention was carried out. These SFC systems showed different selectivites from that found in high-performance liquid chromatography. The association of a constant flow-rate of carbon dioxide and a variable flow-rate of methanol affords the elution of mono-, di- and trisaccharides in the same analysis without baseline drift.
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