Abstract
The sucrose esters of long-chain fatty acids were separated into their components by gel chromatography on Sephadex LH-20. The conditions for gel chromatography were investigated and adjusted as follows: column, 2.0 × 230 cm; column temperature, 50°; developing solvent, DMF; flow rate, 60 ml/h; sample size, 50 mg. Mono-, di-, tri- and higher sucrose esters, sucrose and methyl esters of fatty acids were separated. Reproducibility was satisfactory. The time required for a single determination was about 12 h.
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