Abstract
A high performance liquid chromatographic method has been developed for separation and quantitation of sucrose esters using charged aerosol detection (CAD) combined with mobile phase compensation. Two Acclaim120 C18 columns (75×3.0mm, 3μm) and the gradient composition (0 min – 72% A + 25% B + 3%C, 7.5 min – 75% A + 25% B + 3%C, 19.5 min – 97% A + 3%C, 30 min – 97% A + 3%C, where A is methanol, B is water and C is tetrahydrofuran) were applied. A precisely inverse gradient composition (0 min – 97% A + 3%C, 7.5 min – 97% A + 3%C, 19.5 min – 72% A + 25% B + 3%C, 30 min – 72% A + 25% B + 3%C) was also used. The mobile phase compensation was performed by mixing of the column effluent with the mobile phase of exactly reverse composition provided by a second pump before introduction into the CAD. Introduction
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