Abstract
Analysis of Stem-Cell and Migratory Phenotype in Primary Cultures Derived From BPV-Infected Benign and Malignat Neoplasms
Highlights
Bovine papillomavirus (BPV) is the etiopathogenic agent of bovine papillomatosis, an infectious and neoplastic disease characterized by multiple benign neoplasms that can regress spontaneously, but may persist leading to urinary bladder and esophageal carcinoma when in presence of co-factors [1,2,3,4]
Considering that we first reported genetic and biochemical changes in BPV-infected cells derived from neoplasms, in this study we analyzed the acquisition of cancer stem-cell (CSC) phenotype, as well as the invasiveness capability of primary culture cells derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma
Acquisition of stem-cell phenotype Results of tumorsphere formation assay showed the presence of spheroidal cellular aggregates in primary cell cultures of skin papilloma, fibropapilloma and esophageal carcinoma, but not in normal skin cell line
Summary
Bovine papillomavirus (BPV) is the etiopathogenic agent of bovine papillomatosis, an infectious and neoplastic disease characterized by multiple benign neoplasms (papillomas) that can regress spontaneously, but may persist leading to urinary bladder and esophageal carcinoma when in presence of co-factors [1,2,3,4]. Metastatic process consists of a long series of sequential and interrelated steps in which cancer cells from primary neoplasm spread to distant organs [14] During this process, it is verified biochemical, genetics and morphological changes that epithelial-mesenchymal transition (EMT), a biological reprogramming process which confers migratory and invasiveness capability do transformed cells [6,15]. The lack of studies involving the papillomaviruses (PVs) participation in EMT can be attributed to the little attention given to primary cultures derived from PVs-infected neoplasms In this sense, our group has showed that primary cultures derived from BPV-infected neoplasms are useful models to study the genetic [9] and biochemical deregulations, contributing to genomic instability and cell transformation [7]. Considering that we first reported genetic and biochemical changes in BPV-infected cells derived from neoplasms, in this study we analyzed the acquisition of CSC phenotype, as well as the invasiveness capability of primary culture cells derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma
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