Abstract
Staphylococcus aureus bacteria is a ubiquitous Gram-positive microorganism that causes infections related to the sudden infant death syndrome. Recently, basic detection methods depend on complicated PCR amplification, electric separation, spectric adsorption and other detection systems. However, in this study, simplified sensitive voltammetric skills are developed. To identify an effective diagnostic method for Staphylococcus aureus (SA), a voltammetric sensing probe was sought using mercury immobilized on a carbon nanotube sensor (MCN). The voltammetric MCN conditions were optimized through stripping and cyclic voltammetry. Diagnostic electrolyte was used on non-treated blood sera as an electrolyte solution. The optimum cyclic and stripping analytical working range was 0.5–4.0 mL (3 × 102~5 × 102 CFU/0.5 mL) SA. The statistic relative standard deviation of 0.1 mL SA was observed to be 0.0078 (n = 5). Using the optimum parameters, a diagnostic test was performed by the direct assay of SA in non-treated human blood and patient sera. Here, the developed results can be used for the direct assay of non-treated blood sera, organ monitoring, in-vivo diagnosis, and other assays requiring SA detection.
Highlights
Staphylococcus aureus is a ubiquitous Gram-positive bacterium [1] and a food poisoning agent [2] that causes a variety of infections related to the human toxic shock syndrome and sudden infant death syndrome [3]
External electromagnetic noise was blocked by grounding with a Faraday iron box
Staphylococcus aureus (SA) was maintained on tryptic soy agar slants, grown overnight on soy agar, and continually cultured at 37 ◦C, 24 ± 2 h until a concentration of 109 colony-forming unit (CFU) mL−1 was reached (N = 6)
Summary
Staphylococcus aureus is a ubiquitous Gram-positive bacterium [1] and a food poisoning agent [2] that causes a variety of infections related to the human toxic shock syndrome and sudden infant death syndrome [3]. Advanced diagnostic detection methods were recently developed, including one-step immune chromate graphic assay [4], gold nano particle based immune cromatographic assay [5], real-time nucleicacid-sequence-based amplification assay [6], polymerase chain reaction (PCR) assay [7], real-time PCR assay [8], enzyme-linked immunosorbent assay [9], and multiplex PCR reversed passive latex agglutination (RPLA) identification [10]. Some of these basic methods depend on complicated PCR amplification, electric separation, spectric adsorption and other detection systems demand. In this study, simplified voltammetric methods are tested, which do not require complicated separation, expensive amplification or detection systems
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