Abstract
Soybean is an important cash crop that is widely used as a source of vegetable protein and edible oil. The regeneration ability of soybean directly affects the application of biotechnology. In this study, we used the exogenous hormone 2,4-D to treat immature embryos. Different levels of somatic incidence were selected from the chromosome segment substitution lines (CSSLs) constructed by SN14 and ZYD00006. Transcriptome sequencing of extreme materials was performed, and 2666 differentially expressed genes were obtained. At the same time, a difference table was generated by combining the data on CSSL rearrangement. In the extreme materials, a total of 93 differentially expressed genes were predicted and were then analyzed by cluster analysis and Gene Ontology (GO) annotation. After screening and annotating the target genes, three differentially expressed genes with hormone pathways were identified. The expression patterns of the target genes were verified by real-time quantitative PCR (qRT-PCR). Haplotype polymorphism detection and linkage disequilibrium analysis were performed on the candidate gene Glyma.09g248200. This study provided more information on the regulation network of soybean somatic embryogenesis and regeneration processes, and further identified important genes in the soybean regeneration process and provided a theoretical basis for accelerating the application of biotechnology to soybean for improving its breeding efficiency.
Highlights
Soybean is an important food and oil crop grown all over the world [1]
The results showed that the average incidence of somatic embryos in Suinong 14 and ZYD00006 was 10.88% and 30.27%, respectively, and the incidence of somatic embryos in the offspring of the introduced line ranged from 1% to 71.3% (Table S1)
It is well known that selecting a good set of materials in terms of the genetic population is very important for plant phenotype research
Summary
Soybean is an important food and oil crop grown all over the world [1]. In order to accelerate the soybean breeding process, the application of biotechnology is indispensable. Due to the long genetic transformation cycle of soybean and its low regeneration efficiency, research on soybean gene function is relatively lagging in comparison to other plants. Revealing the mechanism of soybean regeneration is of particular importance for studying the function of soybean genes and accelerating their breeding process. On the 125th anniversary of its publication, Science magazine asked, “How can a single somatic cell form a complete plant?” with the expectation that people would analyze the principle of this phenomenon in future research [2,3]. The process of a single cell developing into a complete plant
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