Abstract
The analysis of amino acids by gas chromatography has become a much more intractable problem than was suspected by the many workers who have tackled it since Bayer’s first demonstration in 1957 [1] that various suitable volatile derivatives could be made and separated. Most of the attention has, naturally, been directed toward the analysis of the amino acids which commonly occur in proteins, since the ion-exchange methods presently available set limitations in sensitivity and speed of analysis. Even with the most recent automatic equipment, the number of analyses that can be done in a day is limited, and maintaining the apparatus takes up scarce technical manpower. Gas chromatography holds out great promise in overcoming these drawbacks, but the accuracy. of the quantitation achieved by ion-exchange chromatography sets a high standard against which to measure gas chromatographic methods, and this has been approached only recently [2].
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