Abstract
The retentions of five β-Lactam antibiotics were analyzed by reversed-phased high-performance liquid chromatography. The mobile phase was acetonitrile/water (15/85, v/v) and the absorption wavelength was determined at 254 nm. When 1-butyl-3-methylimidazolium tetrafluoroborate was added into the mobile phase as additive, the samples were well separated with sharper peaks. The optimal concentration of 1-butyl-3-methylimidazolium tetrafluoroborate at pH 3 was 1.0 mM. The pH has large effect on the retention that the retention factor increasing with the pH of the mobile phase increasing. Three types of 1-alkyl-3-methylimidazolium-based ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, 1-hexyl-3-methylimidazolium tetrafluoroborate, and 1-octyl-3-methylimidazolium tetrafluoroborate) were used and compared as additives in the mobile phase. The results showed that the retention time increased with increasing alkyl chain length. Among the three ionic liquids, the optimal effective additive was 1-butyl-3-methylimidazolium tetrafluoroborate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
