Analysis of Several Popular Cultivars of Madagascar Periwinkle (&lt;i&gt;Catharanthus roseus&lt;/i&gt; (L.) G. Don.) using Biochemical Markers

Owk Aniel Kumar,Sape S Tata,Kancharla Pavan Kumar

doi:10.15835/nsb549178

Abstract

Band designs of esterase (EST), peroxidase (PO) and polyphenol oxidase (PPO) isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE) were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crimson red petal with large white eye’ displayed demarked profiles of EST, PO and PPO isozymes than other cultivars. This is the first report on isozyme polymorphism in members of the Cathanarathus roseus (L.) G. Don.

Highlights

Don. is a perennial plant belonging to the family Apocynaceae and is commonly known as the Madagascar periwinkle was originally classified as Vinca rosea by Linnaeus later it is renamed as Catharanthus roseus based on their morphological features by G
According to the wide literature survey to date on the assessment of genetic diversity of Catharanthus species and cultivars were performed by means of secondary metabolites, AFLP, ISSR, RAPD and RT-SCAR etc. (Chaudhary et al, 2012; El-Domyati et al, 2012; Idrees et al, 2010; Sevestre-Rigouzzo et al, 1993; Shaw et al, 2009)
There has been no previous report on the use of isozyme profiles to characterize the genetic diversity of highly medicinal plant Catharanthus roseus cultivars

Summary

Introduction

There has been no previous report on the use of isozyme profiles to characterize the genetic diversity of highly medicinal plant Catharanthus roseus cultivars. The present study was to investigate the intravarietal relationships by three isozyme profiles (esterase, peroxidase and polyphenol oxidase) from 5 different cultivars of Catharanthus roseus (L.) G.

Results

Conclusion