Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry.

Jingtong Zhao,Tongfei Shi,Yawei Zhao,Fengying Guan,Yuqian Lv,Kan He,Meng Liu,Li Chen,Ming Zhang,Hansi Zhang,Mohan Gao,Jing Li

doi:10.3390/molecules26237181

Jingtong Zhao, Tongfei Shi + Show 10 more

Open Access

https://doi.org/10.3390/molecules26237181

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Abstract

Osteoarthritis is a common multifactorial chronic disease that occurs in articular cartilage, subchondral bone, and periarticular tissue. The pathogenesis of OA is still unclear. To investigate the differences in serum metabolites between OA and the control group, liquid chromatography/mass spectrometry (LC/MS)-based metabolomics was used. To reveal the pathogenesis of OA, 12 SD male rats were randomly divided into control and OA groups using collagenase to induce OA for modeling, and serum was collected 7 days after modeling for testing. The OA group was distinguished from the control group by principal component analysis and orthogonal partial least squares-discriminant analysis, and six biomarkers were finally identified. These biomarkers were metabolized through tryptophan metabolism, glutamate metabolism, nitrogen metabolism, spermidine metabolism, and fatty acid metabolism pathways. The study identified metabolites that may be altered in OA, suggesting a role in OA through relevant metabolic pathways. Metabolomics, as an important tool for studying disease mechanisms, provides useful information for studying the metabolic mechanisms of OA.

Highlights

Osteoarthritis (OA) is an extremely common multifactorial chronic disease that leads to degeneration of joint cartilage, synovial inflammation and osteophyte formation [1]
The phenotypes associated with OA and low-grade inflammation are emerging as new research hotspots in terms of treatment, diagnosis, and prognosis
We investigated the metabolic differences between OA and controls by using a metabolomics approach with liquid chromatography/mass spectrometry (LC/MS)

Summary

Introduction

Osteoarthritis (OA) is an extremely common multifactorial chronic disease that leads to degeneration of joint cartilage, synovial inflammation and osteophyte formation [1]. With the aging of the global population, the pain and disability caused by OA will cause a huge burden on individuals and the social economy [2,3,4]. The most common site for osteoarthritis to occur is the knee joint [5]. The mechanisms by which knee OA occurs are still not fully clear [6]. OA has been considered a disease caused by mechanical damage [7], but an increasing number of studies consider OA to be a low-grade inflammatory disease [8]. The phenotypes associated with OA and low-grade inflammation are emerging as new research hotspots in terms of treatment, diagnosis, and prognosis

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