Analysis of Serum Biochemical Indexes, Egg Quality, and Liver Transcriptome in Laying Hens Fed Diets Supplemented with Gynostemma pentaphyllum Powder.

Tao Li,Jianqin Zhang,Shuya Zhang,Fengwen Xu,Xiuyu Bao,Yiping Song,Jiqiao Zhang

doi:10.3390/genes12121942

Tao Li, Jianqin Zhang + Show 5 more

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https://doi.org/10.3390/genes12121942

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Journal: Genes	Publication Date: Nov 30, 2021
Citations: 2	License type: CC BY 4.0

Affiliation: North West Agriculture and Forestry University

Abstract

Gynostemma pentaphyllum (GP), known as “southern ginseng”, can reduce the blood pressure and blood lipid levels. In this study, 300 layer chicks of one day old were divided randomly into three groups (control group (base diet), high addition group (base diet with 1% GP), and low addition group (base diet with 0.5% GP)). After 29 weeks, the growth performance, egg quality, and serum index were determined. Additionally, liver mRNA was identified using RNA-seq to investigate the molecular mechanisms. The results indicated that the serum total cholesterol and triglycerides decreased significantly in the GP addition group. The addition of GP increased the egg weight, Haugh unit and redness (a*) of the egg yolk color, and reduced the yolk cholesterol concentration. Moreover, 95 differentially expressed genes (DEGs) were screened between the control and GP addition group. GO and the KEGG analysis showed that the PPAR pathway was significantly enriched. Five fatty acid metabolism-related genes (FABP3, CYP7A1, ANKRD22, SCD1, and PCK1) were validated by qRT-PCR analysis, which confirmed the tendency of the expression. These DEGs in the PPAR pathway may be the key factors of GP affecting fatty acid metabolism. These results may provide a theoretical basis for further research and new insights into GP as a feed additive.

Highlights

A dietary supplementation with Gynostemma pentaphyllum (GP) had no effect on the body weight (BW), average daily feed intake (ADFI), ADG, and feed conversion ratio (FCR), which indicated that GP did not affect the growth performance of laying hens
The results showed that the expression of CYP7A1 and ankyrin repeat domain 22 (ANKRD22) was higher Stearoyl-CoA desaturase 1 (SCD1), SCD1, and PCK1 was much lower in the GP addition group than in the control group3)
Gynostemma pentaphyllum (GP) has a lipid-lowering effect on the liver by regulating the key transcriptional factors and lipogenic enzymes involved in fatty acid oxidation during hepatic lipogenesis

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Gynostemma pentaphyllum (GP), called five-leaf ginseng, sweet tea vine, and gospel herb, is a dioecious, herbaceous climbing vine and a member of the Cucurbitaceae family (cucumber or gourd family). GP is widely distributed in South and East Asia and New. Guinea [1] and grows in shady places in the mountains at altitudes of 300–3200 m above sea level. A Chinese plant that is a precious source of saponins, GP does contain eight kinds of gypenosides, which have the same chemical structures as ginsenosides

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