Abstract
Micro-determination of serotonin and histamine was accomplished by means of high-speed liquid chromatography and automated fluorometric analysis using 0- phthaldialdehyde. 0.5 ng of serotonin and 0.1 ng of histamine can be easily deter-mined by the methods described here. For the analysis of urine of healthy human subject, any pre-purification was not carried out to measure serotonin, but in the histamine analysis, extraction by a column of weak cation exchange resin was necessary before injection to the high-speed liquid chromatograph. It was found that diurnal rhythm of urinary serotonin was statistically significant (p<0.01) in healthy human subject, and that the night level of serotonin was slightly higher than the daytime level.
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