Abstract
The retention behavior of selected macrocyclic antibiotics (erythromycin, troleandomycin, tylosin, vancomycin, rifamycin B, and rifampicin) has been examined on LiChrospher Si 60 F 254s HPTLC plates as stationary phase. A wide range of mixtures of alcohols and ketones with hexamethyldisiloxane (HMDSO) in proportions from 0 to 100% ( v/v ) and with dimethyl sulfoxide (DMSO) in proportions from 0 to 50% ( v/v ) were used as mobile phases. Chromatographic retention data and a possible retention mechanism are discussed.
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