Abstract
Quantitatively comparing brain-wide connectivity of different types of neuron is of vital importance in understanding the function of the mammalian cortex. Here we have designed an analytical approach to examine and compare datasets from hierarchical segmentation ontologies, and applied it to long-range presynaptic connectivity onto excitatory and inhibitory neurons, mainly located in layer 2/3 (L2/3), of mouse primary visual cortex (V1). We find that the origins of long-range connections onto these two general cell classes—as well as their proportions—are quite similar, in contrast to the inputs on to a cell type in L6. These anatomical data suggest that distal inputs received by the general excitatory and inhibitory classes of neuron in L2/3 overlap considerably.
Highlights
Comparing brain-wide connectivity of different types of neuron is of vital importance in understanding the function of the mammalian cortex
We show that the input maps onto different cell classes in layer 2/3 (L2/3) are quantitatively more similar to one another than to input maps to a class of principal cells in L6
To target L2/3 glutamatergic, excitatory cells, we instead used a Cre-Off AAV in Gad2-Cre mice (“gadOff ”)[22], so that the AAV was selectively expressed in Gad2-negative, putative excitatory neurons
Summary
Comparing brain-wide connectivity of different types of neuron is of vital importance in understanding the function of the mammalian cortex. Local connectivity shows a high degree of specificity between functionally-related neurons[6,7,9,10,11,12,13] Despite this knowledge of connectivity at a local scale, fewer studies have examined the specificity and pattern of brain-wide, long-range input onto specific cell types in V1, and the majority of those have looked at deeper layers 5 and 615–17. We developed an analytical approach, designed for hierarchical segmentation ontologies, to directly compare these profiles in individual brains Using this approach, we demonstrate that excitatory and inhibitory cells in upper layers receive inputs from the same brain regions, and in similar proportions. We show that the input maps onto different cell classes in L2/3 are quantitatively more similar to one another than to input maps to a class of principal cells in L6
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