ANALYSIS OF SECONDARY METABOLITES OF CALLUS OF RAMBUTAN Nephelium lappaceum L

Elfahmi Elfahmi,Fahrauk Faramayuda,Weni Widy Astuti

doi:10.24246/agric.2021.v33.i1.p13-22

Elfahmi Elfahmi, Fahrauk Faramayuda + Show 1 more

Open Access

https://doi.org/10.24246/agric.2021.v33.i1.p13-22

Copy DOI

Abstract

Rambutan plant (Nephelium lappaceum L.) is a member of the Sapindaceae family. The rambutan plant is one of the natural ingredients that can be developed as traditional medicine. Rambutan peel has the potential for good antioxidant and anticancer activity. Rambutan fruit does not grow every time it needs efforts to produce the active substance in rambutan, using plant tissue culture techniques. The use of the correct variety of mediums and hormones at the right concentration is the key to thriving tissue culture. Explants derived from rambutan leaves were planted precisely on solid media Murashige and Skoog (MS) and WoddyPlant Medium (WPM) containing Indole-3-Butyric Acid (IBA) and Kinetin. After seven days, the callus was subcultured, then after 35 days, the subculture callus was collected and dried. Dry callus and rambutan leaves (Wild type) were macerated with n-hexane, ethyl acetate, and ethanol. The concentrated extract was then applied to a GF 254 silica gel plate with the mobile phase Toluene-Acetone (7: 3) and n-hexane-EthylAsetate (3: 7). The results showed that the concentration of IBA 2 ppm and kinetin three ppm was the best combination because it produced callus. TLC results of rambutan leave with plant tissue culture containing flavonoids and triterpenoids. This study provides new information regarding the induction of rambutan callus and can become the basis for producing active metabolites in rambutan with cell suspension culture development.

Highlights

Rambutan (Nephelium lappaceum L.), belonging to the Sapindaceae family relatives with lychees and longan, are traditional plants used for the treatment of various diseases, including the skin of the fruit for overcome dysentery and fever, bark to overcome thrush,. (Dalimarta, 2003)
Efforts to increase the phenolic compounds that are efficacious as anticancer in the skin of rambutan fruit need to be done with plant tissue culture techniques
Callus culture initiation was carried out using rambutan leaves planted on Murashige and Skoog (MS) and Woody plant medium (WPM) base media with addition (Indole-3 Butyric acid) and kinetin as a growth regulator used in various concentrations to initiate callus culture

Summary

INTRODUCTION

Rambutan (Nephelium lappaceum L.), belonging to the Sapindaceae family relatives with lychees and longan, are traditional plants used for the treatment of various diseases, including the skin of the fruit for overcome dysentery and fever, bark to overcome thrush,. (Dalimarta, 2003). The bark contains tannins, saponins, flavonoids, pectic, and iron (Dalimarta, 2003). Efforts to increase the phenolic compounds that are efficacious as anticancer in the skin of rambutan fruit need to be done with plant tissue culture techniques. Growth regulators (ZPT) are used, which will affect growth and morphogenesis in the cell, tissue, and organ culture, namelyincluding IBA(3-indolebuthylacetic acid), 2,4D (2,4-dichlorophenoxyacetic acid) and NAA (1-naphthyl acetic acid) and kinetin. Do it tothe analysis ofsecondarymetabolite compounds of rambutan leaves by chromatography method Thin Layer. This study expects to know the growth-regulating substances, which are useful for callus induction, and the production of secondary metabolites has activity as medicine from rambutan leaves

MATERIALAND METHODS

And Discussion