Analysis of RPA70N Involvement in RPA ssDNA Binding Activity

Abstract

RPA is the primary single-stranded DNA (ssDNA) binding protein in eukaryotes. It plays a central role in chromosomal DNA replication, repair and recombination pathways, protecting ssDNA from degradation by nucleases. RPA also mediates interactions with specific proteins active in these various DNA processing events. RPA has three subunits, each named after its molecular weight: RPA70 (domains N, A, B and C), RPA32 (domains N, D and C) and RPA14 (single domain). The N-terminal domain of RPA70 (70N) is flexibly linked by an 80 amino acid linker to the rest of RPA 70.