Analysis of River Water Along Klang Valley to Evaluate the Prevalence of Antibiotics Resistant Strains within Urbanized Areas of Selangor, Malaysia

Abstract

The determination of antibiotic-resistant bacteria in Klang river water in Klang valley is performed as the river exposed to various environments. The analysis is performed through enumeration, isolation, and identification process. The water samples were obtained from the origin of the river, housing region, and hospital region. The coliforms obtained through enumeration and identification was then used to determine antibiotic sensitivity, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC). The level of coliforms was indicated through the most probable number (MPN), which 70 MPN per 100 ml of river water in the origin of the river while housing and hospital regions showed more than 1600 MPN per 100 ml of river water. The results obtained from the antibiotic sensitivity test showed that the degree of resistance of coliforms is varied in different regions. The zone of inhibition to ampicillin and tetracyclin for coliforms in housing regions is 20 mm, while the coliforms in the hospital region are 6 mm and 7 mm, respectively. The overall results showed that the level of coliforms and the antibiotic sensitivity of coliforms are different in various regions. The coliforms in the hospital region are more resistant to antibiotics compared to the housing region.