Abstract
This chapter discusses sedimentation velocity boundary analysis of interacting systems that can provide information about stoichiometries and equilibrium constants that is complementary to information obtained by equilibrium sedimentation analysis. It discusses the application of time derivative techniques to the analysis of interacting systems by sedimentation velocity. Potential problems and limitations associated with the computation of weight average sedimentation coefficients for both rapidly reversible and kinetically limited systems in general are treated. The accessible concentration range for sedimentation velocity has been extended considerably by the time derivative/signal averaging method. The combination of taking the time derivative, which eliminates completely the time-independent optical background, and of averaging the time derivative curves results in a considerable increase in precision compared with older methods of analysis. Therefore, because of its sensitivity and selectivity, sedimentation velocity may be the only way the equilibrium constant for an interacting system to be estimated by sedimentation analysis. The chapter also reviews that sedimentation transports of pressure-dependent interacting systems can lead to the generation of negative concentration gradients. In the absence of a stabilizing density gradient, convection can result.
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