Analysis of respiratory viruses detection and clinical feature analysis in children with refractory pneumonia in Lanzhou area

Abstract

Objective The study was to investigation the contribution of 9 respiratory viruses and clinical features of refractory pneumonia in children in Lanzhou area. Methods In total, 100 bronchoalveolar lavage fluid were collected from children younger than 14 years with refractory pneumonia between January 2013 and December 2013. The polymerase chain reaction (PCR) was used to screen human respiratory syncytial virus (HRSV), human metapneumovirus (HMPV), influenza viruses A and B (IFVA, IFVB), parainfluenza virus types 1 to 3 (PIV1-3), human rhinoviruses (HRVs), and human coronaviruses (HCoV-NL63 and HKU1), enterovirus(EV) using a standard reverse-transcription PCR technique, adenovirus(AdVs) and human Bocavirus (Bcov) using traditional PCR methods. All PCR-positive products were sequenced. Results Viral agents were identified in 53% (53/100) of specimens, including AdV in 40%(40/100), followed by RSV in 18%(18/100), IFVB in 5% (5/100), HRV in 4%(4 /100), EV-68 was found in one case. Among AdV positive cases, AdV7 (33/40) was detected most frequently, followed by AdV2 in 15% (6/40), AdV1 in 2.5%(1/40), The coinfection rate of positive sample was 24.53%(13/53). Conclusions Viruses play an important role in refractory pneumonia in children in Lanzhou area. AdV is an important viral agent in children with refractory pneumonia in Lanzhou City, especially AdV7. The frequency of fever, respiratory failure, cyanosis, and pulmonary consolidation were significant between AdV positive sample and negative sample. Key words: Refractory pneumonia; PCR; Viral pathogens; Children