Abstract
Abstract The use of radioactive tracers, analysis of the radioactive compounds by gas chromatography, and measurement of the radioactivity in the effluent of the GLC column represent a powerful combination of techniques that will facilitate the performance of many kinds of studies. The radioactivity can be measured in a number of ways, each of which has been studied, and the experimental conditions necessary for successful operation of each have been defined. Among other applications, this methodology can be used to study biosynthetic pathways in vivo and in vitro, as in fatty acid synthesis, to study relative rates of metabolism of groups of compounds, to determine traces of specific compounds in complex mixtures, and to calibrate the gas chromatograph.
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