Abstract
Melastoma malabathricum L. flowers are part of a plant that contains quercetin and kaempferol. Quercetin and kaempferol are useful for their antioxidant and anticancer properties. The flowering phase affects levels of quercetin and kaempferol. There are four phases of flower blooming: 1st bud phase (K1), 2nd bud phase (K2), 3rd bud phase (K3), and flower bloom (M). It remains to be determined which phase of Melastoma malabathricum L. flowering shows the highest levels of quercetin and kaempferol. Quercetin and kaempferol levels were measured using HPLC MS/MS. The measured quercetin levels of K1, K2, K3, and M phases, respectively, were: 19.47 μg/g, 17.78 μg/g, 31.2 μg/g, and 94.32 μg/g. Levels of kaempferol during K1, K2, K3, and M, respectively, were: 140.99 μg/g, 57.28 μg/g, 95.32 μg/g, and 349.37 μg/g.
Highlights
Banjarbaru South Kalimantan, Indonesia. degasser, quartenary pump, thermostaticalFlowers of M. malabathricum L. are divid- ly controlled autosampler and a personalThe use of natural ingredients for tradi- ed into four phases, namely bud 1 (K1), computer using the program x-calibur 2.1.tional medicine in Indonesia has been com- where flower buds have no petals on dis- Mobile phase A consisted of 0.1% formic mon for centuries
Tional medicine in Indonesia has been com- where flower buds have no petals on dis- Mobile phase A consisted of 0.1% formic mon for centuries
This is evidenced by the play, bud 2 (K2), where flower buds are acid in aquabidest, while phase B consisted old manuscript on palm leaves Husodo starting to show petals, bud 3 (K3), where of 0.1% formic acid in Acetonitrile
Summary
Plant material Purple M. malabathricum L. flowers were used. M. malabathricum L. plant samples were obtained in the morning before 10:00 am from the area of Mount Kupang Banjarbaru South Kalimantan with 30 28 ‘39.02 “S and 1140 51’ 18.69” E. These plants were identified in the Laboratory of Received for publication: 24 August 2016. ©Copyright Isnaini et al, 2018 Licensee PAGEPress srl, Italy International Journal of Plant Biology 2018; 9:6846 doi:10.4081/pb.2018.6846. Extraction M. malabathricum L. flowers were dried at room temperature, mashed. The simplicia obtained was extracted using the maceration method with 96% ethanol and dried with a dirotary evaporator, followed by freeze-drying
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