Abstract
Selenium (Se) is one of the nutrients contained in plants and an essential trace element for human growth. Peanut (Arachis hypogaea L.) is a good material for dietary Se enrichment. To investigate the effects of Se on the soluble proteins from peanut leaves, a comparative proteomics study was performed on soluble peanut leaf proteins (SPLPs), which were extracted from Se-enriched peanut leaves with different Se contents. Then, the allergenicity of the SPLPs was analyzed using in silico approaches. Bioinformatics analysis revealed that low Se treatment activated the antioxidative system and enhanced photosynthesis, glutathione metabolism and primary metabolism. Furthermore, the Bet v 1 domain-containing protein was affected the most by Se enrichment and can be classified as an allergen by docking simulation. ELISA confirmed that the allergenicity of SPLPs was significantly lower than that of the peanut protein, and this lower allergenicity could give the SPLPs a wider range of applications.
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