Abstract
When rice ( Oryza sativa L.) embryos were cultured on Murashige-Skoog medium containing 2,4-dichlorophenoxyacetic acid and kinetin, both embryogenic (E) and non-embryogenic (NE) calli were formed. One and two-dimensional electrophoresis indicated that there were some qualititative differences between the proteins present in E and NE calli, and in rice embryos. Several E and NE-specific and embryo-specific polypeptides were evident. Two of the E-specific polypeptides of 54 and 24 kD corresponded to the major polypeptides in rice embryo extracts. One polypeptide of 22.7 kD was consistently present in the extracts from NE calli, but was present in much lower amounts in E calli, and was absent from embryo extracts. When E and NE calli were placed on regeneration medium for 12 days there were few changes in the polypeptide patterns of the two cell types, except for an increase in the amount of a 45 kD polypeptide in the NE calli.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
