Abstract
Several techniques have been developed, in an effort to minimize sample loss and to improve overall throughput of analysis, that couple liquid chromatography (LC) with mass spectrometry (MS). Coupling microbore HPLC or packed capillary HPLC with electrospray ionization (ESI) on various types of mass analyzers has emerged as the method of choice for the high sensitivity analysis of proteins and peptides. Electrospray ionization has been coupled to several types of mass analyzers. However, ESI on the quadrupole ion trap may prove to be the most powerful tool for the analysis of protein structure. The application of the quadrupole ion trap to the analysis of biomolecules has yielded extraordinary results. Impressive sensitivities have been observed and MSn experiments and ion–molecule reactions have been performed suggesting that the ion trap will become an important tool for protein analysis. This chapter demonstrates the use of ESI for the analysis of proteins. The techniques used involved coupling packed capillary HPLC and open-tubular liquid chromatography with ESI on a triple quadrupole mass spectrometer and on a quadrupole ion trap.
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