Analysis of predicted B and T-cell epitopes in Der p 23, allergen from Dermatophagoides pteronyssinus.

Songwe Fanuel,Gholam Ali Kardar,Saeideh Tabesh,Esmaeil Sadroddiny

doi:10.6026/97320630013307

Songwe Fanuel, Gholam Ali Kardar + Show 2 more

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https://doi.org/10.6026/97320630013307

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Journal: Bioinformation	Publication Date: Sep 30, 2017
Citations: 8	License type: cc-by

Affiliation: Tehran University of Medical Sciences

Abstract

House dust mite (HDM) allergy is the leading cause of IgE-mediated hypersensitivity. Therefore identifying potential epitopes in the Dermatophagoide pteronyssinus 23 (Der p 23), a major house dust mite allergen will aid in the development of therapeutic vaccines and diagnostic kits for HDM allergy. Experimental methods of epitope discovery have been widely exploited for the mapping of potential allergens. This study sought to use immunoinformatic methods to analyze the structure of Der p 23 for potential immunoreactive B and T-cell epitopes that could be useful for AIT and allergy diagnosis. We retrieved a Der p 23 allergen sequence from Genbank database and then analyzed it using a combination of web-based sequence analysis tools including the Immune Epitope Database (IEDB), Protparam, BCPREDS, ABCpred, BepiPred, Bcepred among others to predict the physiochemical properties and epitope spectra of the Der p 23 allergen. We then built 3D models of the predicted B-cell epitopes, T cell epitopes and Der p 23 for sequence structure homology analysis. Our results identified peptides 'TRWNEDE', 'TVHPTTTEQPDDK', and 'NDDDPTT' as immunogenic linear B-cell epitopes while 'CPSRFGYFADPKDPH' and 'CPGNTRWNEDEETCT' were found to be the most suitable T-cell epitopes that interacted well with a large number of MHC II alleles. Both epitopes had high population coverage as well as showing a 100% conservancy. These five Der p 23 epitopes are useful for AIT vaccines and HDM allergy diagnosis development.

Highlights

House dust mites (Dermatophagoides pteronyssinus (Der p)) are among the most important etiologic agents of IgE-mediated allergy
At least 20 Der p allergens from House dust mite (HDM) have been identified, with Der p 1, Der p 2 and Der p 11 being classified as major allergens [1]
Physiochemical and secondary structure analysis: The primary sequence of Der p 23 used for this analysis contained 69 amino acids

Summary

Introduction

House dust mites (Dermatophagoides pteronyssinus (Der p)) are among the most important etiologic agents of IgE-mediated allergy. At least 20 Der p allergens from HDM have been identified, with Der p 1, Der p 2 and Der p 11 being classified as major allergens (showing sensitization in more than 50% of patients) [1]. HDM IgE-mediated allergic reactions occur after a sensitized patient comes in contact with one or more HDM groups of allergens, resulting in an overproduction of Der p-specific IgE antibodies. The symptoms of IgE-mediated diseases range from mild allergic rhinitis, dermatitis, conjunctivitis, sometimes to life threatening anaphylaxis and allergic asthma [2]. It has been demonstrated that allergen immunotherapy (AIT) is the only effective way of treatment that addresses the underlying mechanisms of IgEmediated reactions. To find new remedial alternatives, AIT development strategies are centered on identifying epitopes responsible for allergic responses and designing of appropriate hypoallergenic AIT vaccines [5]

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