Abstract
Figure 1: Comparison of elution of a peptide in its unmodified and modified form Extracted ion chromatograms are shown for two peptides in unmodified form and with post translational modification using Spectronaut. The upper graph shows the extracted ion current of the fragments. The lower graphs show the MS1 signal of the peptide with its isotopic pattern. The dotted line shows the expected retention time stemming from the spectral library as determined with iRT. The green area indicates the integration area for the peaks. Note the coelution of the MS1 and the MS2 signals.
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