ANALYSIS OF POLYMORPHISMS OF RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM AND RELATION TO VASOPRESSORS IN ESSENTIAL SYSTEMIC HYPERTENSION WITH THE LEFT VENTRICLE HYPERTROPHY IN DAGESTAN REPUBLIC

Abstract

Aim. To assess the prevalences of genotypes and alleles polymorphisms of reninangiotensin-aldosterone system, and β2-adrenoreceptors, and to evaluate the results in regard to vasopressor levels in blood serum in essential systemic hypertension (ESH) with the left ventricle hypertrophy (LVH) and with none, in Dagestan Republic population. Material and methods. To the assessment, 98 patients included with the diagnosis “essential systemic hypertension with or none LVH”. Genotypes were assessed, of polymorphism A1166C gene AGTR1 and polymorphism Arg16Gly gene ADRB2. The testing of the polymorphisms was performed with allele-specific polymerase chain reaction. Level of angiotensin (At) II, endothelin (et) 1-21 and aldosterone (AS) in blood serum was measured by the hard-phase IeA. Level of ACe was measured by enzymatic method. Statistics was done with the software statistica (version 6.0) and “Biostat 4.03”. Results. In EAH patients with LVH the prevalence of Arg/Arg polymorphism Arg16Gly gene ADRB2 was lower than in control. In EAH with no LVH there is significant decline of AC genotype prevalences of polymorphism A1166C gene AGTR1 and genotype Arg/Arg polymorphism Arg16Gly gene ADRB2. Level of EТ1-21 and АТ II in the group of EAH with no LVH patients was significantly higher than in controls. Level of AT II in EAH with LVH was significantly higher than none LVH group. Increase of the levels of these mediators of AH was followed by a decline of AS level in the groups of patients comparing to controls. In EAH group with LVH significant increase of et 1-21 was associated with the carriage of As polymorphism A1166C gene AGTR1, and genotypes АА and СС of the same polymorphism were associated with the decrease of levels of EТ1-21. In EAH with no LVH a significant decrease of At II level was found in the carriers of AA and AC genotypes of polymorphism A1166C gene AGTR1. Also, the association was found of all genotypes of polymorphism Arg16Gly gene ADRB2 with the decrease of АТ II. Conclusion. In Dagestan population, in EAH with and with no LVH, most significant are genotypes AA, AC and CC of polymorphism A1166C gene AGTR1, and genotypes Arg/Arg, Arg/Gly, Gly/Gly and alleles Arg and Gly polymorphism Arg16Gly gene ADRB2. Pathogenic significance of the mentioned polymorphisms is emphasized by the presence of polymorphism ET1-12, АТ II, Аs in blood serum.