Abstract
The lily (Lilium spp.) anther contains a lot of pollen. It is not known if lily pollen contains allergens, and therefore screening pollen allergy-related proteins and genes is necessary. The pollen development period of lily ‘Siberia’ was determined by microscope observation. Early mononuclear microspores and mature pollens were used as sequencing materials. The analysis of the pollen transcriptome identified differentially expressed genes (DEGs), e.g., Profilin, Phl p 7 (Polcalcin), Ole e 1, and Phl p 11, which are associated with pollen allergens. The proteome analysis positively verified a significant increase in pollen allergenic protein content. The expression levels of LoProfiilin and LoPolcalcin, annotated as allergen proteins, gradually increased in mature pollen. LoProfiilin and LoPolcalcin were cloned and their open reading frame lengths were 396 bp and 246 bp, which encoded 131 and 81 amino acids, respectively. Amino acid sequence and structure alignment indicated that the protein sequences of LoProfilin and LoPolcalcin were highly conserved. Subcellular localization analysis showed that LoProfilin protein was localized in the cell cytoplasm and nucleus. LoProfilin and LoPolcalcin were highly expressed in mature pollen at the transcriptional and protein levels. A tertiary structure prediction analysis identified LoProfilin and LoPolcalcin as potential allergens in lily pollen.
Highlights
(Lilium spp.) is a perennial herbaceous bulbous flower of the Liliaceae, which has ornamental, medicinal, and edible functions [1,2]
The results showed that the secondary structure of LoProfilin contained 26.72% alpha helix, 24.43% extended strand, 7.63% beta turn, and 41.22% random coil, while LoPolcalcin contained 62.96% alpha helix, 6.17% extended strand, 12.35% beta turn, and 18.52% random coil
The analysis of the pollen transcriptome identified differentially expressed genes (DEGs), e.g., Profilin, Phl p 7 (Polcalcin), Ole e 1, and Phl p 11, which were associated with pollen allergens
Summary
(Lilium spp.) is a perennial herbaceous bulbous flower of the Liliaceae, which has ornamental, medicinal, and edible functions [1,2]. The anthers of lily are huge and contain a large amount of pollen; when the flowers open, pollen pollution will cause many problems, such as clothing contamination, and could affect the health of people [4,5]. Pollen is one of the main factors determining the occurrence of seasonal allergies. During the period of flower opening, pollen grains are released into the air to form biological aerosols; individuals are inevitably exposed to pollen [7]. Pollen pollution seriously affects the quality of life for people susceptible to allergies. There have been few studies of the allergic proteins in ornamental plants, especially lily
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
