Abstract

In vitro production (IVP) of embryo is one of the most propitious assisted reproductive technology (ART) adopted in modern livestock industry. Bangladesh, a developing country has been working enormously to adopt this technique in various livestock species. As goat is the most promising small ruminant of this country, several research work has been ongoing to adopt IVP on this species, more specifically on Black Bengal goat (BBg), the only recognized breed of Bangladesh. For conservation, improvisation and increased production of this goat breed ART like IVP is a major pre requisite. IVP of embryos usually includes retrieval of oocytes from the ovarian follicles, in vitro maturation (IVM), sperm capacitation, in vitro fertilization (IVF) of oocytes and in vitro culture (IVC) of presumptive zygotes to morula or blastocyst stage. Research showed, the source and quality of the ovary, oocyte and the cumulus-oocyte-complexes (COCs), semen quality, oocyte recovery time and procedure, culture condition, supplements and the subsequent factors plays a critical role in the maturation and subsequent development of the embryo which can be varied from lab too lab upon researcher’s choice. A short review on the IVP of goat embryos is discussed here in the context of recent encouraging data which will aid future researchers aiming at successful embryo production of BBg in vitro.

Highlights

  • In vitro production of embryo is an emerging concern in the livestock industry today

  • Regardless oocyte recovery technique, efficacy of oocytes recovery assessed based on which type of goat used for oocyte collection and observed that, greater amount of COCs recovered from pubertal Black Bengal goat (BBG) (88%) than from their prepubertal (77%) counterparts (Khatun et al, 2011)

  • Prior to in vitro maturation (IVM), the morphology of the cumulus investment is widely used as a selection criterion, as it has a significant impact on the maturity of goat oocytes

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Summary

INTRODUCTION

In vitro production of embryo is an emerging concern in the livestock industry today. Researchers have been trying to determine which conditions are needed during IVM, IVF, and in vitro development (IVD) processes (Joanna Maeia et al, 2014) and which supplementation and positive factors are beneficial to enhance embryo production to optimize the technique, allowing increasing its adoption in Bangladesh in near future. This is an short review of the research works done on Black Bengal. Goat which would be an aid in future research works regarding IVP of goat embryos

Oocyte retrieval
Selection and grading of retrieved oocytes
In vitro maturation of oocytes
In vitro fertilization and in vitro culture
Effect of follicular fluid
Right and left ovary
Age of the goat
Findings
CONCLUSIONS
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