Analysis of platelet deposition and binding of anti-fibrinogen antibody to the artificial surfaces preadsorbed with sera or plasmas from patients with normal and elevated CRP

Abstract

Background: As far as fibrinogen (Fb) is an essential protein in mediating platelet adhesion in vitro, the colorimetric adhesion assay can differentiate platelet deposition to normal plasma from fibrinogen-deficient plasma in healthy donors. However, it is not known whether it may discriminate platelet response to serum and/or plasma originating from patients with and without the increased inflammatory activity. Aims: In the present study we compared ADP-induced platelet adhesion in healthy subjects to serum or plasma from patients with normal and increased CRP concentration (CRP>5 mg/L). Also, the binding of anti-Fb antibodies to serum and plasma from patients with varying CRP concentrations was examined. Methods: For microplate coating, both serum and plasma from patients with varying CRP concentrations were utilized. Colorimetric assays were used to determine platelet adhesion and anti-Fb antibody binding. Results: We demonstrated significantly higher platelet adhesion to the sera and plasmas from patients with elevated CRP compared to platelet adhesion to the sera and plasmas from patients with normal CRP. Also, we observed significantly higher anti-Fb binding to the sera from patients with elevated CRP compared to anti-Fb binding to the sera from patients with normal CRP. Furthermore, the binding of anti-Fb antibody to the sera of patients significantly correlated with CRP concentration. Conclusions: Overall, this study supports the data on the existence of a strong relation between CRP and fibrinogen level and it leads to the conclusion that the adhesion assay may be a useful tool in differentiating platelet response to sera and plasmas from patients with and without the increased inflammatory activity.