Abstract

SummaryWe compared sequence variations in the internal transcribed spacer (ITS) region of five Lens species, including cultivated lentil (Lens culinaris subsp. culinaris) and its four wild relatives distributed in Iran and Turkey, in order to clarify their genetic relationships. Cluster analysis based on the neighbour-joining algorithm and Jukes-Cantor distances revealed a phenetic tree with a topology congruent with patterns derived by previous studies using ITS sequence analysis. Phylogenetic analysis confirmed the divergence of L. nigricans from all other species. In this study, we found that some accessions of L. culinaris subsp. orientalis were very similar to L. culinaris subsp. culinaris, the cultivated lentil, but several others were not.

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