Analysis of Phthalide, Fenobucarb and Inabenfide in Unpolished Rice Using Selective Gel Permeation Chromatography

Abstract

We studied a simple cleanup method using selective gel permeation chromatography (GPC) for the analysis of phthalide, fenobucarb and inabenfide in unpolished rice. These pesticides in ground unpolished rice were extracted with acetonitrile. Acetonitrile layer was separated by salting-out with NaCl. The residue was loaded to GPC (acetone–cyclohexane, 20 : 80, v/v). After fenobucarb, inabenfide and phthalide were fractionated by GPC, the each eluant was determined by GC-NPD, HPLC and GC-ECD, respectively, without further cleanup. Average recoveries (n=5) of fenobucarb, inabenfide and phthalide from fortified rice were 98, 90 and 81% at the 0.2 μg/g level and the limit of detection was 0.01 ppm, 0.01 ppm and 0.005 ppm, respectively.