Analysis of PDCD1 gene copy number differences between hepatocellular carcinoma tissues and paracancerous tissues of patients with HBV-related HCC

Abstract

Objective To analyze the differences in the PDCD1 gene copy number of hepatocellular carcinoma and paracancerous tissues in patients with hepatocellular carcinoma(HCC)after HBV infection. Methods Analysis PDCD1 gene copy number of 27 paraffin embedded specimens of hepatocellular carcinoma tissues and para carcinoma tissues of patients with hepatocellular carcinoma after resection by Taqman real-time PCR. Results The gene copy number of PDCD1 of hepatocellular carcinoma tissue and paracancerous tissue had no significant difference (P>0.05); the gene copy number of PDCD1 and preoperative AFP levels of hepatocellular carcinoma and paracancerous tissues had no significant correlation (P>0.05). Gene copy number of PDCD1 of hepatocellular carcinoma and paracancerous tissues had no significant correlation with tissue tumor malignant degree (P>0.05). Conclusions PD-1 plays a role in the escape response of tumor immunity, but the PDCD1 gene copy number variation of hepatocellular carcinoma and paracancerous tissues is not a factor in carcinogenesis and development of hepatocellular carcinoma. Key words: PD-1; PDCD1; Hepatocellular carcinoma; Gene; Variation