Analysis of patrilineal relationship in Russian Federation using several commercial Y-STR multiplex panels

Abstract

We have analysed 285 cases of patrilineal relationship between individuals from various regions of the Russian Federation. Y-STR analysis was performed using Yfiler®, Yfiler® Plus (both Applied Biosystems, USA), and COrDYS-Y (GORDIZ Ltd, Russia) genotyping systems. The number of meioses between the individuals varied from 1 to 3 (based on self-declaration by the study participants). In 235 cases either a complete match of Y-STR profiles between alleged relatives (86.0%) or mismatches at no more than 3 loci (14.0%) were observed. In 50 cases, more than 3 mismatches between participants were detected and these samples where not taken for further analysis. When using Yfiler® (n=119) a complete match between participants was observed in 91.6% cases, 1 mismatch – in 7.6% cases, and 3 mismatches – in 0.8% cases. When using Yfiler® Plus (n=58) a complete match between participants was observed in 79.3% cases, 1 mismatch – in 20.7% cases, and 2 mismatches – in 3.4% cases. In cases analysed by COrDYS-Y (n=58) a complete match between participants was observed in 81.0% cases, 1 mismatch – in 13.8% cases, 2 mismatches – in 3.5% cases, and 3 mismatches – in 1.7% cases. All mismatches detected during the present study were by one STR repeat with a single exception of locus DYS627 (Yfiler® Plus) where a mismatch of two repeats was found in one case. Overall, mismatches were present at all loci studied with exception of DYS392, DYS437, DYS438, DYS447, DYS448, DYS481 and DYS533. The highest mutation frequency was observed for locus DYS570 (0.0256).