Abstract
In the past few years, short tandem repeat (STR) typing has become the method of choice for many, if not most, laboratories to perform paternity testing. The aim of this study was to show that a set of carefully chosen and well-known STR loci may provide as reliable results as Restriction Fragment Length Polymorphism (RFLP) typing does. We analyzed the Paternity Index (PI) and Residual Paternity Index (RPI) obtained in 67 non-exclusion cases and 22 exclusion cases typed by 10 STR loci, and in 61 non-exclusions and 14 exclusions typed by four RFLP loci. PI was calculated for the trios and also for child and alleged father in motherless cases using local frequency tables and it was assigned to one out of six categories. The 10 STR locus analysis for paternity testing led to conclusive results for all trio cases. In paternity tests lacking a mother, more than 10 STRs should be analyzed to get similar results.
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