Analysis of organic acids in fruit-bodies of Tricholoma giganteum by high performance liquid chromatography

Abstract

A high performance liquid chromatographic method (HPLC) was developed for the determination of organic acids (oxalic, tartaric, formic, L-malic, lactic, acetic, α-ketoglutaric, DL-pyroglutamic, citric, succinic and fumaric acids) in the edible mushroom Tricholoma giganteum. Organic acid contents were 0.01 to 0.10% on a dry weight basis and seven kinds of organic acids (oxalic, malic, acetic, α-ketoglutaric, pyroglutamic, succinic and fumaric acids) were identified. Oxalic and succinic acids were found to be predominant in the mushroom.