Abstract
Abstract The method described involves passing an alcoholic extract of fruit product through a cation exchange resin column and then through an anion exchange resin column. Neutral fruit components are washed from the columns with alcohol and acetone solutions. Acidic components trapped on the anion column are eluted using 6N formic acid in acetone. An aliquot of the eluate is evaporated to dryness after the addition of benzene to remove the excess formic acid as an azeotrope. The residue is allowed to react with bis(trimethylsilyl)acetamide or bis(trimethylsilyl)trifluoroacetamide to form trimethylsilyl ether-ester derivatives which are detected by gas chromatography on a nonpolar (OV-1) column. Good recoveries were obtained for some common organic acids (succinic, fumarie, malic, tartaric, trans- aconitic, and citric acids) from fruit juice.
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