Analysis of optically anisotropic properties of biological tissues under stretching based on differential Mueller matrix formalism.

Hao-Wei Chen,Chih-Ling Huang,Yu-Lung Lo,You-Ren Chang

doi:10.1117/1.jbo.22.3.035006

Abstract

The optical properties of biological tissues under stretching are investigated using a full-field ellipsometry technique based on a differential Mueller matrix formalism. Traditional photoelastic-based formalism for extracting the linear birefringence (LB) properties of stretched anisotropic optical samples ignores the effects of the other optical properties of the sample. By contrast, in the formalism proposed in this study, the LB, linear dichroism (LD), circular birefringence (CB), circular dichroism (CD), and depolarization (Dep) properties are fully decoupled. Simulations are performed to evaluate the performance of the two formalisms in extracting the LB properties of optically anisotropic samples with different degrees of Dep, CB, LD, and CD. The practical feasibility of the proposed all-parameter decoupled formalism is then demonstrated using chicken breast muscle tissue. In general, the results show that both formalisms provide a reliable LB measurement performance for healthy chicken breast tissue under stretching. However, while the LB-only formalism has good robustness toward scattering, its measurement performance is seriously degraded for samples with high CB. Thus, of the two formalisms, the proposed all-parameter decoupled formalism provides a more effective approach for examining the anisotropic properties of biological tissues under stretching.

Highlights

Biological tissues have unique and well-documented microscopic fibrous structures, which result in a pronounced birefringence property.[1]
To evaluate the resulting error in the extraction results, a series of simulations was performed in which the linear birefringence (LB) results obtained using the LB-only model were compared with those obtained using the all-parameter decoupled model for four hypothetical anisotropic samples with hybrid (LB + Dep), (LB + circular birefringence (CB)), (LB + linear dichroism (LD)), and (LB + circular dichroism (CD)) properties, respectively
Type 2 diabetes may translate into functional impairment in the elder people and this may reflect a link between the mechanical functions of muscle[22,23] and the muscle birefringence (LB) is due to the well-arranged muscle structures

Summary

Introduction

Biological tissues have unique and well-documented microscopic fibrous structures, which result in a pronounced birefringence property.[1] The change in these structures under stretching results in a change in the birefringence. Liao et al.[4] used a CCD camera and Monte Carlo simulations to analyze the anisotropic optical properties of chicken heart tissue. They developed a rotating linear polarization imaging technique and obtained a set of new images of the parameters by fitting linear differential polarization (LDP) images pixel-by-pixel to an analytical expression. The observation results revealed that the heart muscles are aligned in different directions at different sections of the organ, and the heart exhibits a strong

Methods

Results

Conclusion