Abstract
Thermostable glycoprotein amyloid bioemulsifier BE-AM1 was reported from Solibacillus silvestris AM1. S. silvestris could produce better BE-AM1 in 1% inoculum size and 18h inoculum age. The BE-AM1 production by S. silvestris AM1 has been seen to be unaffected by amending the production and growth medium (Zobel Marine medium) with Carbon and Nitrogen sources. The effects of 16 nutrient factors (from ZM medium) were analyzed statistically by one factor at a time, Plackett-Burman and Response surface methodologies. Among the nutritive factors, yeast extract and peptone were found to be significant, increasing the production quantity of BE-AM1. The time course of production of BE-AM1 was affected significantly by altering the release of the biomolecule from the surface of the bacterium. This will be the first study to analyse the effect of factors influencing the production of industrially important functional amyloid protein from bacterial source having potential in environmental remediation and microbial interaction.
Highlights
The high molecular weight amphipathic biosurfactants are termed as Bioemulsifiers which act as capsular polymers that are released into environment by the producing strain during growth (Amiriyan, et al 2004)
The amyloids produced due to the mis-folding of the cellular proteins in mammalian tissues resulting in neurodegenerative diseases in hosts are termed pathological amyloids while their purposeful production in fungi and bacteria for specific physiological functions of the producing cell are known as functional amyloids (Blanco, et al, 2012; Nielsen rt al., 2011)
As the objective of present study were to elucidate the nutritive factors influencing the production of amyloid bioemulsifier BEAM1 using statistical methods like Plackett Burman and Response surface methodology
Summary
The high molecular weight amphipathic biosurfactants are termed as Bioemulsifiers which act as capsular polymers that are released into environment by the producing strain during growth (Amiriyan, et al 2004). S. silvestris AM1 was reported previously for the production of amyloid bioemulsifiers BE-AM1 having the ability to produce stable emulsions in aliphatic and aromatic hydrocarbons (Markande et al, 2013). As the objective of present study were to elucidate the nutritive factors influencing the production of amyloid bioemulsifier BEAM1 using statistical methods like Plackett Burman and Response surface methodology. This will be the first report on the statistical analysis of nutrient influences on the production of bacterial functional amyloid having surfactant properties with potential applications in food industries
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