Abstract
Abstract An HPLC method for the analysis of nitrofurantoin (NTR) in human plasma has been developed and evaluated. This method is based on a selective extraction and reverse-phase chromatography which results in chromatograms with 2400 to 2500 theoretical plates for NTR and the internal standard (furazolidone). The method is highly reproducible and has been validated over the range of 5 to 4000 ng/ml. The HPLC method developed in our laboratories for the analysis of nitrofurantoin is specific and sensitive to 5 ng/ml. When samples are pipetted at the time of collection or preparation, concentrations up to 4000 ng/ml can be assayed reproducibly with precision. Concentrations above 4000 ng/ml would require dilution prior to analysis. Samples are stable at least 10 days when frozen at -157deg;C. Assays by this method of over 200 samples from subjects receiving both intravenous and oral doses of nitrofurantoin have demonstrated it to be both reproducible and rugged.
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